Molecular Biology Core Equipment

Next Generation Sequencing:

""

Illumina Next Seq 550 Next Generation Sequencer

  • Targeted DNA sequencing
  • Whole transcriptome (RNA) sequencing
  • Metagenomic sequencing
  • ChIP (chromatin IP) sequencing
  • Transcriptome sequencing
  • Small RNA sequencing
  • For more information please contact Pamela Plant
  • Book Now

""

Covaris Focused Ultrasonicator

The Invitrogen Qubit 4 Fluorometer is designed to quickly and specifically quantitate DNA or RNA used in library prep for NGS

Gene Expression Profiling:

Nanostring553

NanoString

  • Highly multiplexed gene expression profiling
  • No amplification or reverse transcription of mRNA is required as probes read directly off sample mRNA
  • Compatible with RNA extracted from FFPE samples
  • For more information please contact Pamela Plant
  • Book Now

""

BioRad QX200 Digital Droplet PCR for gene expression profiling

  • Provides absolute quantification of target DNA or RNA molecules
  • Reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required
  • Optimal for gene expression analysis of small fold changes for low abundance mRNA and miRNA targets
  • For more information please contact Pamela Plant
  • Book Now

QuantStudio 7 Real-Time PCR System

  • Real Time PCR machine for absolute and relative quantitation of gene expression
  • 384 well format
  • Newer version of ViiA 7 – same software
  • For more information please contact Pamela Plant
  • Book Now

ViiA7qPCR

ABI ViiA7

  • Blocks available for 384, 96 Fast & TaqMan Low Density Array formats
  • Contact Pamela Plant for list of associated reagents
  • Book Now

Eppendorf Electronic Multidispense Pipettes

Eppendorf Electronic Multidispense Pipettes

  • For RNA/cDNA use only
  • P2-10 and P10-100 available
  • Booking calendar in room 553

Captair RNA/DNA Rolling Clean Hood

Captair RNA/DNA Rolling Clean Hood

  • Laminar flow hood with UV light
  • On metallic rolling cart with locking wheels

Quality Assessment:

Bioanalyzer

Agilent 2100 Bioanalyzer

  • Nano, pico and microRNA analysis
  • DNA high sensitivity analysis
  • Book Now

""

Qubit Flurometer

Routine Molecular Biology Equipment:

ABI Veriti Thermocycler

ABI Veriti Thermocycler

ChemiDocTouch550

BioRad ChemiDoc Touch CCD Imager for Western Blots

  • Supports the acquisition of images from a wide range of gels and blots using a supersensitive camera with a charge-coupled device (CCD)
  • Large maximum aperture lens that provides high chemiluminescent sensitivity and wide dynamic range
  • Digital images are processed in Image lab software where quantitation of Westerns  is possible with and without Stain free technology
  • Book Now

LiCorOdyssey550

Li-Cor Odyssey Infrared Imager for Western Blots

  • Ability to quantitatively detect two targets in the same sample lane on Western blot
  • Infrared fluorophores enable quantitative protein detection
  • Wide linear range with sensitivity equal to or better than that of chemiluminescence
  • Book Now

SynergyNeo550

BioTek Synergy NEO Multimode Plate Reader

  • Gen 5 software drives the acquisition and analysis of multiple assay types and detection modes on the machine including: absorbance, fluorescence, luminescence, time resolved fluorescence (TRF), fluorescence polarization (FP) and Alpha technology assays
  • For more information please contact Pamela Plant
  • Book Now

Eppendorf Mastercycler Gradient Thermocycler:

  • Beckman Allegra centrifuge for 96, 384, TLDA plates
  • Book Now

BioRad GelDoc Easy gel documentation system:

  • Specialty service offering Lecia CM 3050S Cryostat, ArcturusXT PiXCell Laser capture Microdissection (LCM) System, Leica LCM
  • Book Now
X