The Nikon inverted widefield/fluorescent microscope has a Xenon fluorescent light source and a Hamamatsu Orca ER monochrome digital CCD camera for image capture. It has Plan Fluor lenses with phase contrast and differential interference contrast capabilities.

Lenses        
objective type mag N.A. W.D.mm
Plan Fluor 4 0.13 17.1
Plan Fluor Ph1 10 0.30 15.2
Plan Fluor ELWD Ph1 20 0.45 8.1-7.0
Plan Fluor ELWD Ph2 40 0.60 3.1-2.7
Plan Apo oil 60 1.40 0.21

 

Filter cubes  
descrip Ex nm Em nm
DAPI 395 460/50
‘green’ 485 535/25
‘red’ 560 610/75
X